Biological indicator Geobacillus Stearothermophilus

The biological indicator with suspension contains viable bacteriene spores of Geobacillus Stearothermophilus, for verification of sterilization in the autoclave. The presence of Geobacillus stearothermophilus spores is detected by a visual color change (turns yellow). The yellow color change indicates a sterilization process failure. The final readout of a negative result (remains purple) is made after 48 hours of incubation. It is compatible with conventional incubators. Instructions for use: 1. Identify the biological indicator by writing the sterilizer, load number, and processing date on the indicator label. 2. Place a biological indicator horizontal or cap up in an appropriate test tray or package according to recommended practices. 3. Place the test tray or package in a full load in the most challenging area for the sterilant. This is generally on the bottom shelf, near the door and over the drain. 4. Process the load as usual. 5. After the completion of the cycle and while wearing safety glasses and thermal gloves, fully open the sterilizer door for a minimum of 5 minutes prior to removing the biological indicator. 6. When the biological indicator is not contained in a test package or other heat absorbing packaging material, remove the biological indicator from the sterilizer and allow to cool for an additional 10 minutes prior to crushing. 7. While wearing safety glasses, crush and incubate the biological indicator at 56 ± 2°C (133 ± 3°F). 8. Incubate processed and control biological indicators for 48 hours at 56 ± 2°C (133 ± 3°F). 9. The appearance of a yellow color in the processed indicator demonstrates bacterial growth and a sterilization process failure. No color change indicates an adequate sterilization process. A final negative result is made after 48 hours of incubation. The positive control indicator should show a yellow color change for the processed indicator results to be valid.